Simple • Affordable • Next Generation Sequencing
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Technologies
We take pride in our rigorous development and validation processes, resulting in the creation of three highly effective ClickSeq-based NGS library preparation approaches:
ClickSeq™: RNAseq without the fragmentation or ligation.
ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3′-Azido cDNAs:
ClickSeq was originally published in 2015 in the Journal of Molecular Biology as a method to make RNAseq libraries that do not contain artifactual chimeric reads to allow the detailed analysis of rare recombination events in RNA viruses.
Poly(A)-ClickSeq: 3' end RNAseq for gene expression and polyadenylation analysis
Poly(A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation:
A method for sequencing just the 3′ ends of eukaryotic messenger RNAs by priming from poly(A) tails and using AzATP, AzGTP and AzCTP to terminate RT-PCR just upstream of the poly(A) tail in the 3’ UTR was published in Nucleic Acids Research in 2017.
Tiled-ClickSeq: Targeted sequencing with only one template-specific primer per amplicon
Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants:
Tiled-ClickSeq leverages the ClickSeq™ approach to perform complete genome or gene sequencing. Using multiple tiled primers, overlapping amplicons spanning the target are generated. Tiled-ClickSeq has been optimized for the whole genome sequencing of RNA viruses including SARS-CoV-2.
Advantages of ClickSeq™:
No fragmentation required;
No enzymatic ligation;
Ultra-low artifactual chimera formation¹;
Specialized Transcriptomic applications;
Integrated analysis pipelines, from RNA to results;
Affordable library synthesis
RNA or DNA
Suitable for fragmented/degraded samples
Request our services or contact us: info@clickseq.com
Recent Publications and Highlights
Sung, P.-y.; Zhou, Y.; Kao, C. C.; Aburigh, A. A.; Routh, A.; Roy, P.,
A multidisciplinary approach to the identification of the protein–RNA connectome in double-stranded RNA virus capsids.
de Prisco, N.; Ford, C.; Elrod, N. D.; Lee, W.; Tang, L. C.; Huang, K.-L.; Lin, A.; Ji, P.; Jonnakuti, V. S.; Boyle, L.,
Alternative polyadenylation alters protein dosage by switching between intronic and 3′ UTR sites.
Johnson, B. A.; Xie, X.; Bailey, A. L.; Kalveram, B.; Lokugamage, K. G.; Muruato, A.; Zou, J.; Zhang, X.; Juelich, T.; Smith, J. K.,
Loss of furin cleavage site attenuates SARS-CoV-2 pathogenesis.
