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ClickSeq Publications
ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3′-Azido cDNAs:
ClickSeq was originally published in 2015 in the Journal of Molecular Biology as a method to make RNAseq libraries that do not contain artifactual chimeric reads to allow the detailed analysis of rare recombination events in RNA viruses.
Sung, P.-y.; Zhou, Y.; Kao, C. C.; Aburigh, A. A.; Routh, A.; Roy, P.,
A multidisciplinary approach to the identification of the protein–RNA connectome in double-stranded RNA virus capsids.
Sotcheff, S.; Zhou, Y.; Yeung, J.; Sun, Y.; Johnson, J. E.; Torbett, B. E.; Routh, A. L.,
ViReMa: a virus recombination mapper of next-generation sequencing data characterizes diverse recombinant viral nucleic acids.
Radnaa, E.; Urrabaz-Garza, R.; Elrod, N. D.; de Castro Silva, M.; Pyles, R.; Han, A.; Menon, R.,
Generation and characterization of human Fetal membrane and Decidual cell lines for reproductive biology experiments.
Mascibroda, L. G.; Shboul, M.; Elrod, N. D.; Colleaux, L.; Hamamy, H.; Huang, K.-L.; Peart, N.; Singh, M. K.; Lee, H.; Merriman, B.,
INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex.
Funk, O. H.; Qalieh, Y.; Doyle, D. Z.; Lam, M. M.; Kwan, K. Y.,
Postmitotic accumulation of histone variant H3. 3 in new cortical neurons establishes neuronal chromatin, transcriptome, and identity.
Soto-Acosta, R.; Edwards, T. C.; Dreis, C. D.; Krishna, V. D.; Cheeran, M. C.; Qiu, L.; Xie, J.; Bonnac, L. F.; Geraghty, R. J.,
Enhancing the Antiviral Potency of Nucleobases for Potential Broad-Spectrum Antiviral Therapies.
Langsjoen, R. M.; Zhou, Y.; Holcomb, R. J.; Routh, A. L.,
Chikungunya Virus Infects the Heart and Induces Heart-Specific Transcriptional Changes in an Immunodeficient Mouse Model of Infection.
Doyle, D. Z.; Lam, M. M.; Qalieh, A.; Qalieh, Y.; Sorel, A.; Funk, O. H.; Kwan, K. Y.,
Chromatin remodeler Arid1a regulates subplate neuron identity and wiring of cortical connectivity.
Zhou, Y.; Routh, A.,
Mapping RNA–capsid interactions and RNA secondary structure within virus particles using next-generation sequencing.
Langsjoen, R.; Muruato, A.; Kunkel, S.; Jaworski, E.; Routh, A.,
Differential alphavirus defective RNA diversity between intracellular and extracellular compartments is driven by subgenomic recombination events.
Keil, J. M.; Doyle, D. Z.; Qalieh, A.; Lam, M. M.; Funk, O. H.; Qalieh, Y.; Shi, L.; Mohan, N.; Sorel, A.; Kwan, K. Y.,
Symmetric neural progenitor divisions require chromatin-mediated homologous recombination DNA repair by Ino80.
Kautz, T. F.; Jaworski, E.; Routh, A.; Forrester, N. L.,
A low fidelity virus shows increased recombination during the removal of an alphavirus reporter gene.
Huang, K.-L.; Jee, D.; Stein, C. B.; Elrod, N. D.; Henriques, T.; Mascibroda, L. G.; Baillat, D.; Russell, W. K.; Adelman, K.; Wagner, E. J.,
Integrator recruits protein phosphatase 2A to prevent pause release and facilitate transcription termination.
Shi, L.; Qalieh, A.; Lam, M. M.; Keil, J. M.; Kwan, K. Y.,
Robust elimination of genome-damaged cells safeguards against brain somatic aneuploidy following Knl1 deletion.
Reyes-Ruiz, J. M.; Osuna-Ramos, J. F.; Bautista-Carbajal, P.; Jaworski, E.; Soto-Acosta, R.; Cervantes-Salazar, M.; Angel-Ambrocio, A. H.; Castillo-Munguía, J. P.; Chávez-Munguía, B.; De Nova-Ocampo, M.,
Mosquito cells persistently infected with dengue virus produce viral particles with host-dependent replication.
Elrod, N. D.; Henriques, T.; Huang, K.-L.; Tatomer, D. C.; Wilusz, J. E.; Wagner, E. J.; Adelman, K.,
The integrator complex attenuates promoter-proximal transcription at protein-coding genes.
Jaworski, E.; Routh, A.,
ClickSeq: replacing fragmentation and enzymatic ligation with click-chemistry to prevent sequence chimeras.
Jaworski, E.; Routh, A.,
Parallel ClickSeq and Nanopore sequencing elucidates the rapid evolution of defective-interfering RNAs in Flock House virus.
Routh, A.; Head, S. R.; Ordoukhanian, P.; Johnson, J. E.,
ClickSeq: fragmentation-free next-generation sequencing via click ligation of adaptors to stochastically terminated 3′-azido cDNAs.
Routh, A.; Chang, M. W.; Okulicz, J. F.; Johnson, J. E.; Torbett, B. E.,
CoVaMa: Co-Variation Mapper for disequilibrium analysis of mutant loci in viral populations using next-generation sequence data.