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 Poly(A)-ClickSeq Publications

Read Coverage over Human Akt1 mRNA: Poly(A)-ClickSeq (PAC-Seq) vs RNAseq

Poly(A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation: 

Routh et al. NAR 2017

A method for sequencing just the 3′ ends of eukaryotic messenger RNAs by priming from poly(A) tails and using AzATP, AzGTP and AzCTP to terminate RT-PCR just upstream of the poly(A) tail in the 3’ UTR was published in Nucleic Acids Research in 2017. 

Mukherjee, S.;  Graber, J. H.; Moore, C. L., 

Macrophage differentiation is marked by increased abundance of the mRNA 3’end processing machinery, altered poly (A) site usage, and sensitivity to the level of CstF64. 

Mimoso, C. A.; Adelman, K., 

U1 snRNP increases RNA Pol II elongation rate to enable synthesis of long genes. 

Jonnakuti, V. S.;  Ji, P.;  Gao, Y.;  Lin, A.;  Chu, Y.;  Elrod, N.;  Huang, K.-L.;  Li, W.;  Yalamanchili, H. K.; Wagner, E. J., 

NUDT21 alters glioma migration through differential alternative polyadenylation of LAMC1. 

de Prisco, N.;  Ford, C.;  Elrod, N. D.;  Lee, W.;  Tang, L. C.;  Huang, K.-L.;  Lin, A.;  Ji, P.;  Jonnakuti, V. S.; Boyle, L., 

Alternative polyadenylation alters protein dosage by switching between intronic and 3′ UTR sites. 

Vu, M. N.;  Lokugamage, K. G.;  Plante, J. A.;  Scharton, D.;  Bailey, A. O.;  Sotcheff, S.;  Swetnam, D. M.;  Johnson, B. A.;  Schindewolf, C.; Alvarado, R. E., 

QTQTN motif upstream of the furin-cleavage site plays a key role in SARS-CoV-2 infection and pathogenesis. 

Sotcheff, S. L.;  Chen, J. Y.-C.;  Elrod, N.;  Cao, J.;  Jaworski, E.;  Kuyumcu-Martinez, M. N.;  Shi, P.-Y.; Routh, A. L., 

Zika Virus Infection Alters Gene Expression and Poly-Adenylation Patterns in Placental Cells. 

LaForce, G. R.;  Farr, J. S.;  Liu, J.;  Akesson, C.;  Gumus, E.;  Pinkard, O.;  Miranda, H. C.;  Johnson, K.;  Sweet, T. J.; Ji, P., 

Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration. 

Scarborough, A. M.;  Flaherty, J. N.;  Hunter, O. V.;  Liu, K.;  Kumar, A.;  Xing, C.;  Tu, B. P.; Conrad, N. K., 

SAM homeostasis is regulated by CFIm-mediated splicing of MAT2A. 

Montalbano, M.;  Jaworski, E.;  Garcia, S.;  Ellsworth, A.;  McAllen, S.;  Routh, A.; Kayed, R., 

Tau modulates mRNA transcription, alternative polyadenylation profiles of hnRNPs, chromatin remodeling and spliceosome complexes. 

Li, L.;  Huang, K.-L.;  Gao, Y.;  Cui, Y.;  Wang, G.;  Elrod, N. D.;  Li, Y.;  Chen, Y. E.;  Ji, P.; Peng, F., 

An atlas of alternative polyadenylation quantitative trait loci contributing to complex trait and disease heritability. 

Enwerem, I. I.;  Elrod, N. D.;  Chang, C.-T.;  Lin, A.;  Ji, P.;  Bohn, J. A.;  Levdansky, Y.;  Wagner, E. J.;  Valkov, E.; Goldstrohm, A. C., 

Human Pumilio proteins directly bind the CCR4-NOT deadenylase complex to regulate the transcriptome. 

Li, L.;  Huang, K.-L.;  Gao, Y.;  Cui, Y.;  Wang, G.;  Elrod, N. D.;  Li, Y.;  Chen, Y. E.;  Ji, P.; Peng, F., 

An atlas of alternative polyadenylation quantitative trait loci contributing to complex trait and disease heritability. 

Cao, J.;  Verma, S. K.;  Jaworski, E.;  Mohan, S.;  Nagasawa, C. K.;  Rayavara, K.;  Sooter, A.;  Miller, S. N.;  Holcomb, R. J.; Powell, M. J., 

RBFOX2 is critical for maintaining alternative polyadenylation patterns and mitochondrial health in rat myoblasts. Cell reports 2021, 37 (5) 

Alcott, C. E.;  Yalamanchili, H. K.;  Ji, P.;  van der Heijden, M. E.;  Saltzman, A.;  Elrod, N.;  Lin, A.;  Leng, M.;  Bhatt, B.; Hao, S., 

Partial loss of CFIm25 causes learning deficits and aberrant neuronal alternative polyadenylation. 

Routh, A., 

DPAC: A Tool for Differential Poly (A)–Cluster Usage from Poly (A)–Targeted RNAseq Data. 

Elrod, N. D.;  Jaworski, E. A.;  Ji, P.;  Wagner, E. J.; Routh, A., 

Development of Poly (A)-ClickSeq as a tool enabling simultaneous genome-wide poly (A)-site identification and differential expression analysis. 

Chu, Y.;  Elrod, N.;  Wang, C.;  Li, L.;  Chen, T.;  Routh, A.;  Xia, Z.;  Li, W.;  Wagner, E. J.; Ji, P., 

Nudt21 regulates the alternative polyadenylation of Pak1 and is predictive in the prognosis of glioblastoma patients. 

Routh, A.;  Ji, P.;  Jaworski, E.;  Xia, Z.;  Li, W.; Wagner, E. J., 

Poly (A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation. 

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