Simple • Affordable • Next Generation Sequencing
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We provide Next-Generation Sequencing services from sample to data.
Tailored projects and flow-throughs to meet your specific needs.
Library Synthesis – Starting at only $175 per library prep.
We offer RNAseq, DNAseq, Poly(A)-targeted and template-targeting Next-Generations Sequencing services based upon our patented and proprietary ClickSeq library synthesis approaches. We can either return completed NGS libraries to users, or proceed to sequencing on Illumina platforms.
For full pricing and quotes, please contact us at info@clickseq.com
Sequencing on Illumina platforms
For full pricing and quotes, please contact us at info@clickseq.com
Bioinformatics support and analysis
We have extensive experience providing bioinformatics, processing and analysis of NGS data. This includes routine activities such as data QC, adaptor trimming and quality filtering as well as more specific applications such as read alignment, genomics, transcriptomics, differential gene expression analysis, alternative polyadenylation analysis and virus diversity analysis.
For full pricing and quotes, please contact us at info@clickseq.com
For advanced projects or bespoke analyses that require custom script writing and/or project-specific expertise, please contact us at info@clickseq.com
Submission Requirements
Please find here details for sample submission for regular Library Preparation strategies. If these conditions cannot be met, please consult us for options or for other custom/targeted approaches (info@clickseq.com).
Ideally, samples should be provided in individual low-bind nuclease-free tubes or plates. For large projects with >24 samples, please provide in plate format.
Samples should be shipped to our facility using over-night services, preferably on dry-ice.
ClickSeq_WhitePage_v1.2.pdfRNAseq (random-primed ClickSeq)
RNAseq
Recommended:
250 ngs of Purified RNA (e.g. poly(A) selected RNA, viral genomic RNA, etc)
Concentration = 10ng/µl or greater
A260/A280 1.9-2.2
Provided in nuclease-free water (must be free of residual ethanol)
No RNA Fragmentation
RIN>6.0
Do not use carrier RNA during RNA purification
Minimum:
>50 ngs of purified RNA;
Concentration = 5ng/µl or greater
If fragmented and/or degraded, please consult first and provide estimate of RNA fragment lengths.
PACSeq_WhitePage_v1.2.pdfPoly(A)-ClickSeq
Recommended:
>1000 ngs of Purified Total Cellular RNA
Concentration = 10ng/µl or greater
A260/A280 = 1.9-2.2
Provided in nuclease-free water (must be free of residual ethanol)
No RNA Fragmentation
No RNA Enrichment/Selection
RIN>6.0
IMPORTANT: Do not use carrier RNA during RNA purification (this is usually poly(A)-oligomers.
Minimum:
>50 ngs of Purified Total Cellular RNA
Concentration = 5ng/µl or greater
If fragmented and/or degraded, please consult first and provide estimate of RNA fragment lengths.
Tiled-ClickSeq
Recommended:
Input requirements very depending upon sample origin, target type and target abundance. Please reach out at info@clickseq.com to discuss.
Custom primer design may be necessary. Please reach out at info@clickseq.com to discuss.
A260/A280 = 1.9-2.2
Provided in nuclease-free water (must be free of residual ethanol)
No RNA Fragmentation
RIN>6.0
If fragmented and/or degraded, please consult first and provide estimate of RNA fragment lengths.
DNAseq (Random-primed ClickSeq)
Recommended:
>500 ngs of Purified dsDNA or ssDNA
Concentration = 10ng/µl or greater
A260/A280 > 1.7
Preferably provided in water, TE or other common elution buffer (no residual ethanol)
No DNA fragmentation
Minimum:
>50 ngs of Purified DNA
Concentration = 5ng/µl or greater
Small circular DNAs (e.g. plasmids) may need to be linearized (e.g. by restriction digest)
If fragmented and/or degraded, please consult first and provide estimate of DNA fragment lengths.
