No fragmentation required;
No enzymatic ligation;
Ultra-low artifactual chimera formation¹;
Specialized Transcriptomic applications;
Integrated analysis pipelines, from RNA to results;
Affordable library synthesis
RNA or DNA
Suitable for fragmented/degraded samples
ClickSeq™
RNAseq without the fragmentation or ligation.
ClickSeq was originally published in 2015 in the Journal of Molecular Biology as a method to make RNAseq libraries that do not contain artifactual chimeric reads to allow the detailed analysis of rare recombination events in RNA viruses.
Poly(A)-ClickSeq
3' end RNAseq for simplified gene expression and alternative polyadenylation analysis
A method for sequencing just the 3′ ends of eukaryotic messenger RNAs by priming from poly(A) tails and using AzATP, AzGTP and AzCTP to terminate RT-PCR just upstream of the poly(A) tail in the 3’ UTR was published in Nucleic Acids Research in 2017.
Tiled-ClickSeq
Targeted sequencing: Each tiled amplicon is derived from only one template-specific primer
Tiled-ClickSeq leverages the ClickSeq™ approach to perform complete genome or gene sequencing. Using multiple tiled primers, overlapping amplicons spanning the target are generated. Tiled-ClickSeq has been optimized for the whole genome sequencing of RNA viruses including SARS-CoV-2.